General

• DNA Quantitation: Fluorometric vs Spectrophotometric Methods
• Group or crowd sequencing
• How do you design gene panels?
• How long does genome sequencing take?
• How long will you store my sample or extraction?
• How to access your sequencing data
• NCBI Nucleotide Blast Search
• Sample submission and shipping guidelines
• Types of sequencing services
• What does your pricing include?
• What is The Sequencing Center's biosafety rating?
• What's included in "basic bioinformatics"?
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Genome Sequencing

• Complete Genome Assembly
• How do you quantify DNA concentration?
• What are paired-end reads?
• What organisms can you sequence?

HLA Typing

• HLA IMGT database
• HLA NGSengine
• HLA nomenclature
• HLA publication list for GenDx
• HLA sample requirements
• How to read HLA reports
• What does an HLA report look like?

Whole Genome Bioinformatics

• Can I use one of my sequenced samples as a reference genome?
• Genome Assembly with PATRIC
• How to read FastQ files
• How to use Phaster for bacteriophage data analysis
• Reference genomes
• Trimming Illumina adapter sequences
• What are unmapped reads?
• What does a sequence alignment and variant call report look like?
• What is de novo assembly?
• What is sequencing coverage?
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Metagenomic Bioinformatics

• Microbiome reports with Kraken
• Microbiome reports with Megan6
• Microbiome reports with One Codex

Documentation

• Protocol documents and manuals

Geneious Bioinformatics

• Geneious sequence alignment & variant calls workflow
• Genome annotation with Geneious
• How to create Geneious variant call reports
• How to read Geneious variant call reports
• Sequence alignments with Geneious