General

• What does your pricing include?
• What is multiplex sequencing?
• How long does genome sequencing take?
• Protocol documents and manuals
• What is The Sequencing Center's biosafety rating?

Sample Preparation

• Sample submission and shipping guidelines
• DNA Quantitation: Fluorometric vs Spectrophotometric Methods
• How much DNA is contained in human blood cells?
• How long will you store my sample or extraction?
• DNA Concentration Protocol

Genome Sequencing

• How to find a reference genome
• Can I use one of my sequenced samples as a reference genome?
• How to generate a complete genome with hybrid assembly
• What are paired-end reads?
• What is sequencing coverage?

HLA Typing

• HLA sample requirements
• What does an HLA report look like?
• How to read HLA reports
• HLA nomenclature
• HLA IMGT Database
• HLA publication list for GenDx

Bioinformatics

• Microbial Bioinformatics
  • Geneious sequence alignment & variant calls workflow
  • Sequence alignments with Geneious
  • How to create Geneious variant call reports
  • How to read Geneious variant call reports
  • How to do genome annotation with Geneious
  • How to use Phaster for bacteriophage bioinformatics
• Whole Genome Bioinformatics
  • How to read FastQ files
  • Trimming Illumina adapter sequences
  • What are unmapped reads?
  • What does a sequence alignment and variant call report look like?
  • What is de novo assembly?
  • How to perform de novo assembly with PATRIC
• Metagenomic Bioinformatics
  • How to read One Codex microbiome reports
  • Microbiome Reports with Kraken
  • Microbiome reports with Megan6

• How to access your sequencing data
• What's included in "basic bioinformatics"?
• How to Perform an NCBI Nucleotide Blast Search

Gene Panels

• How do you design gene panels?
• How to read the Alzheimer's gene panel reports