HLA-DRB1, HLA-DRB3, HLA-DRB4 and HLA-DRB5 are difficult to type for several reasons. First, these four HLA alleles are highly polymorphic, meaning that they can have multiple variations that make it difficult to accurately type them. Second, these HLA alleles are located close together on human Chromosome 6, making it difficult to distinguish between them. Third, the allele sequences of are very similar, which can make it difficult to differentiate between them.
The difficulty in typing these HLA alleles is further compounded by the fact that they are not easily detected using molecular methods. Molecular methods such as PCR require a known sequence to be used as a reference to detect HLA alleles. However, due to their high degree of polymorphism, it can be difficult to find a reference sequence that accurately matches the allele that is being typed.
Lastly, the difficulty in typing these four HLA alleles is also due to the fact that they are not easily detected using serological methods. Serological methods such as ELISA require the presence of specific antibodies to accurately detect HLA alleles. However, due to high polymorphism, it can be difficult to find antibodies that can accurately detect them.
In conclusion, HLA-DRB1, HLA-DRB3, HLA-DRB4 and HLA-DRB5 are difficult to type due to their high polymorphism, their close location on chromosome 6, the similarity of their allele sequences, the difficulty in finding reference sequences for molecular methods, and the difficulty in finding antibodies for serological methods.
The Sequencing Center is one of the leading service providers for HLA Typing and Whole Genome Sequencing (WGS) in the USA. Offering affordable, high-coverage HLA typing results with 4-field resolution and microbial scale WGS, we provide clients with exceptional services, including DNA extraction, library prep, sequencing and bioinformatics. We work with leading research organizations world-wide in various fields including immunology, oncology, transplantation, inherited diseases, neurodegenerative diseases, and drug discovery and development.
1020 Luke Street
Suite D
Fort Collins, CO 80524
U.S.A.
(877) 425-2235
(970) 682-1288 (Direct to lab)
info@thesequencingcenter.com
This site uses cookies. By continuing to browse the site, you are agreeing to our use of cookies.
OKLearn moreWe may request cookies to be set on your device. We use cookies to let us know when you visit our websites, how you interact with us, to enrich your user experience, and to customize your relationship with our website.
Click on the different category headings to find out more. You can also change some of your preferences. Note that blocking some types of cookies may impact your experience on our websites and the services we are able to offer.
These cookies are strictly necessary to provide you with services available through our website and to use some of its features.
Because these cookies are strictly necessary to deliver the website, refusing them will have impact how our site functions. You always can block or delete cookies by changing your browser settings and force blocking all cookies on this website. But this will always prompt you to accept/refuse cookies when revisiting our site.
We fully respect if you want to refuse cookies but to avoid asking you again and again kindly allow us to store a cookie for that. You are free to opt out any time or opt in for other cookies to get a better experience. If you refuse cookies we will remove all set cookies in our domain.
We provide you with a list of stored cookies on your computer in our domain so you can check what we stored. Due to security reasons we are not able to show or modify cookies from other domains. You can check these in your browser security settings.
These cookies collect information that is used either in aggregate form to help us understand how our website is being used or how effective our marketing campaigns are, or to help us customize our website and application for you in order to enhance your experience.
If you do not want that we track your visit to our site you can disable tracking in your browser here:
We also use different external services like Google Webfonts, Google Maps, and external Video providers. Since these providers may collect personal data like your IP address we allow you to block them here. Please be aware that this might heavily reduce the functionality and appearance of our site. Changes will take effect once you reload the page.
Google Webfont Settings:
Google Map Settings:
Google reCaptcha Settings:
Vimeo and Youtube video embeds:
The following cookies are also needed - You can choose if you want to allow them:
You can read about our cookies and privacy settings in detail on our Privacy Policy Page.
Privacy Policy