Phaster is a bioinformatics tool for analyzing bacteriophage sequencing data sets. It generates a variety of phage identification and annotation results that are very useful for interpreting phage sequencing data. Currently, Phaster contains about 187K viral sequences and 9M bacterial sequences, making it a very comprehensive database for phage research.
The Sequencing Center will provide you with FastA formatted de novo assembly files. These files will be available in your Box.com account in an “Assembly” directory. The assembly filenames will include the text “…..scaffolds.fasta”. You will use the assembly files in Phaster.
For most bacteriophage data sets, Phaster will run for a few minutes. The tool will display run progress in a separate webpage. When Phaster is done it displays a “Submission Results” page. The results are fairly extensive and cannot be covered in detail in this short note. Please see the Phaster Help webpage and Phaster Ouput webpage for more information about how to interpret the results.
The Sequencing Center is one of the leading service providers for HLA Typing and Whole Genome Sequencing (WGS) in the USA. Offering affordable, high-coverage HLA typing results with 4-field resolution and microbial scale WGS, we provide clients with exceptional services, including DNA extraction, library prep, sequencing and bioinformatics. We work with leading research organizations world-wide in various fields including immunology, oncology, transplantation, inherited diseases, neurodegenerative diseases, and drug discovery and development.
1020 Luke Street
Suite D
Fort Collins, CO 80524
U.S.A.
(877) 425-2235
(970) 682-1288 (Direct to lab)
info@thesequencingcenter.com
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