Phaster is a bioinformatics tool for analyzing bacteriophage sequencing data sets. It generates a variety of phage identification and annotation results that are very useful for interpreting phage sequencing data. Currently, Phaster contains about 187K viral sequences and 9M bacterial sequences, making it a very comprehensive database for phage research.
The Sequencing Center will provide you with FastA formatted de novo assembly files. These files will be available in your Box.com account in an “Assembly” directory. The assembly filenames will include the text “…..scaffolds.fasta”. You will use the assembly files in Phaster.
For most bacteriophage data sets, Phaster will run for a few minutes. The tool will display run progress in a separate webpage. When Phaster is done it displays a “Submission Results” page. The results are fairly extensive and cannot be covered in detail in this short note. Please see the Phaster Help webpage and Phaster Ouput webpage for more information about how to interpret the results.
The Sequencing Center is a USA-owned and operated next-generation genome sequencing company offering affordable genome sequencing and bioinformatics for research, pharmaceutical, and clinical organizations. Our services are designed for organizations performing research on bacterial, viral, and human-oriented research. Among these services, our facility offers targeted sequencing methods for research fields including inherited diseases, oncology, neurodegenerative diseases, antibiotic-resistant gene analysis, and biomarker discovery for drug development optimization.
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