The non-classical HLA-G allele is associated with numerous disease states, including pre-eclampsia, asthma, several types of cancer and autoimmune diseases. We sequence HLA-G using a whole-gene amplification strategy from GenDx. In HLA-G there are several well-known polymorphisms in the 3′-UTR region that may contribute to various diease conditions, hence, we are seeing increased interest in sequencing HLA-G specifically to examine 3′-UTR mutations.
The GenDx technote “HLA-G typing including 3′ UTR polymorphisms” describes a 14-bp indel (dbSNP ID rs66554220) and nine SNP’s in HLA-G 3′-UTR that we can view with NextGen Sequencing protocols. As part of this process we use the bioinformatics tool NGSengine from GenDx for analysis of this region.
Figures 1 – 4 show four possible 14-bp indels at locus 2961 (rs66554220) – deletion/deletion, insertion/insertion, deletion/insertion and insertion/deletion, respectively.
Fig. 1. 14-bp deletion/deletion at locus 2961.
Fig. 2. 14-bp insertion/insertion at locus 2961.
Fig. 3. 14-bp deletion/insertion at locus 2961.
Fig. 4. 14-bp insertion/deletion at locus 2961.
These well-known indels are easily identified in NGSengine and captured in our client reports.
Table 1. Sample HLA-G report.
We deliver a standardized report for the HLA-G allele based upon the nomenclature defined in the GenDx technote “HLA-G typing including 3′ UTR polymorphisms”.
The report includes:
If you’re interested in HLA-G typing and in particular the 3′-UTR region, please contact us for more information and quotes for service.
The Sequencing Center is one of the leading HLA service providers in the USA. Offering affordable, high-coverage HLA typing results with 4-field resolution, we pride ourselves on providing clients with exceptional services, including DNA extraction, library prep, sequencing and bioinformatics. We work with leading research organizations world-wide across a variety of fields including immunology, oncology, transplantation, inherited diseases, neurodegenerative diseases, and drug discovery and development.
1020 Luke Street
Fort Collins, CO 80524
(970) 682-1288 (Direct to lab)
Click on the different category headings to find out more. You can also change some of your preferences. Note that blocking some types of cookies may impact your experience on our websites and the services we are able to offer.
These cookies are strictly necessary to provide you with services available through our website and to use some of its features.
We provide you with a list of stored cookies on your computer in our domain so you can check what we stored. Due to security reasons we are not able to show or modify cookies from other domains. You can check these in your browser security settings.
These cookies collect information that is used either in aggregate form to help us understand how our website is being used or how effective our marketing campaigns are, or to help us customize our website and application for you in order to enhance your experience.
If you do not want that we track your visit to our site you can disable tracking in your browser here:
We also use different external services like Google Webfonts, Google Maps, and external Video providers. Since these providers may collect personal data like your IP address we allow you to block them here. Please be aware that this might heavily reduce the functionality and appearance of our site. Changes will take effect once you reload the page.
Google Webfont Settings:
Google Map Settings:
Google reCaptcha Settings:
Vimeo and Youtube video embeds:
The following cookies are also needed - You can choose if you want to allow them: