We use the GenDx NGSengine application for HLA typing and HLA report generation.
The HLA loci DRB3, DRB4 and DRB5 are paralogs of locus DRB1. Thus, they share a significant fraction of their genetic sequence with DRB1. The NGSengine HLA…
https://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.png00Deidre Caseyhttps://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.pngDeidre Casey2021-05-18 13:49:042021-05-18 15:01:34Why does DRB1/3/4/5 show ‘Insufficient data’?
The IPD-IMGT/HLA database contains genetic sequence and allele data for the human major histocompatibility complex (MHC). This database is foundational for many HLA Typing algorithms and Next-Generation sequencing projects. It…
We vet our vendors carefully before using products in our sequencing workflows. The Sequencing Center uses the GenDx libraries for NGS (Next Generation Sequencing)-based HLA typing. The combination of substantial immunological research,…
https://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.png00Richard Caseyhttps://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.pngRichard Casey2019-07-08 11:07:092021-05-19 07:50:33HLA publication list for GenDx
We deliver detailed HLA reports to clients immediately after their HLA sequencing runs are complete. The reports include information about HLA allele typing calls, allele ambiguities, and relevant metadata.
The full set of HLA loci…
https://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.png00Richard Caseyhttps://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.pngRichard Casey2019-05-14 15:46:332021-05-19 05:49:56How to read HLA reports
The standardized HLA gene and allele nomenclature is managed by the WHO Nomenclature Committee for Factors of the HLA System. A high-level summary of their nomenclature system is reproduced here for easy reference. A thorough and detailed…
We use GenDx NGSgo and NGSengine for HLA projects. The minimum sample requirements for this HLA typing assay are:
DNA mass: 1,800 ng
DNA concentration: 25-30 ng/µL (measured by Qubit fluorometer)
Sample volume: 60 µL
The Sequencing Center is a USA-owned and operated next-generation genome sequencing company offering affordable genome sequencing and bioinformatics for research, pharmaceutical, and clinical organizations. Our services are designed for organizations performing research on bacterial, viral, and human-oriented research. Among these services, our facility offers targeted sequencing methods for research fields including inherited diseases, oncology, neurodegenerative diseases, antibiotic-resistant gene analysis, and biomarker discovery for drug development optimization.
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