What is the read length for short-read genome sequencing?
We use Illumina MiniSeq for short-read sequencing runs. The paired-end short read lengths are always 2 x 150bp = 300bp. Our library prep protocols are designed to fragment DNA into 150bp reads and paired-end reads combine two reads (forward and reverse) into a single 300bp read.
Note that paired-end reads always include an “insert” sequence with a modal distribution peak around 300bp. Effectively, this yields a short-read read length around 600bp.
https://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.png00Deidre Caseyhttps://thesequencingcenter.com/wp-content/uploads/2019/01/TSC_wide_logo_blue-e1546560904499.pngDeidre Casey2021-03-24 03:57:062022-12-22 08:30:56What is the read length for short-read genome sequencing?
The Sequencing Center is one of the leading service providers for HLA Typing and Whole Genome Sequencing (WGS) in the USA. Offering affordable, high-coverage HLA typing results with 4-field resolution and microbial scale WGS, we provide clients with exceptional services, including DNA extraction, library prep, sequencing and bioinformatics. We work with leading research organizations world-wide in various fields including immunology, oncology, transplantation, inherited diseases, neurodegenerative diseases, and drug discovery and development.
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