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What is the read length for short-read genome sequencing?

We use an Illumina MiniSeq for our short-read sequencing runs.  The paired-end short read lengths are always 2 x 150bp = 300bp.  The library prep protocols are designed to fragment DNA into 150bp reads, and paired-end read runs combine two…

What are paired-end reads?

Many sequencing library preparation kits include an option to generate so-called "paired-end reads".  In "short-read" sequencing, intact genomic DNA is sheared into several million short DNA fragments called "reads".  Individual reads can…