What is the read length for short-read genome sequencing?
We use an Illumina MiniSeq for our short-read sequencing runs. The paired-end short read lengths are always 2 x 150bp = 300bp. The library prep protocols are designed to fragment DNA into 150bp reads, and paired-end read runs combine two…
What are paired-end reads?
Many sequencing library preparation kits include an option to generate so-called "paired-end reads". In "short-read" sequencing, intact genomic DNA is sheared into several million short DNA fragments called "reads". Individual reads can…