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What is the FastQ file naming convention?

When your FastQ files are delivered, you will notice they have a specific file name pattern. This is due to the naming requirements we must abide by for our bioinformatics software. In order to determine which of your samples is which, reference…
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What is the read length for short-read genome sequencing?

We use an Illumina MiniSeq for our short-read sequencing runs.  The paired-end short read lengths are always 2 x 150bp = 300bp.  The library prep protocols are designed to fragment DNA into 150bp reads, and paired-end read runs combine two…

What are paired-end reads?

Many sequencing library preparation kits include an option to generate so-called "paired-end reads".  In "short-read" sequencing, intact genomic DNA is sheared into several million short DNA fragments called "reads".  Individual reads can…
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What is sequencing coverage?

Coverage is defined as the number of sample nucleotide bases sequence aligned to a specific locus in a reference genome.  The easiest way to explain this is with a real sequenced bacterial sample that has been aligned to the reference genome…
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What is multiplex sequencing?

Some researchers may only have one or two samples that need to be sequenced. However, with the price of consumables and facility overhead, sequencing a single sample is often cost-prohibitive in normal sequencing lab settings. Multiplex sequencing…