What is index hopping?
Index hopping occurs when index (barcode) sequences initially assigned to a specific sample are incorrectly assigned to other sample(s) in a pool of samples. The result of index hopping is the incorrect assignment of sample reads (DNA fragments)…
What is index dropout?
In Illumina sequencing, indexing (barcoding) is the process of adding unique tags to each read in a specific sample. The indexing step is performed during library preparation after the genomic fragmentation step. After fragmentation, each…
What is the FastQ file naming convention?
When your FastQ files are delivered, you will notice they have a specific file name pattern. This is due to the naming requirements we must abide by for our bioinformatics software. In order to determine which of your samples is which, reference…
Do you offer sequencing discounts?
Yes! There are a couple of ways you save even more on your research budget.
Pre-pay Discount
Any project that is paid in full upfront will receive a 3% discount on the total project price.
Volume Discount
Any project with >96 samples…
What is the read length for short-read genome sequencing?
We use an Illumina MiniSeq for our short-read sequencing runs. The paired-end short read lengths are always 2 x 150bp = 300bp. The library prep protocols are designed to fragment DNA into 150bp reads, and paired-end read runs combine two…
Where to find a genetic counselor
We offer HLA (human leukocyte antigen) sequencing services for immune system and histocompatibility research. As part of this service we deliver HLA reports that include allele typing for up to 12 HLA genes. Genetic counselors are available…
What are paired-end reads?
Many sequencing library preparation kits include an option to generate so-called "paired-end reads". In "short-read" sequencing, intact genomic DNA is sheared into several million short DNA fragments called "reads". Individual reads can…
What is sequencing coverage?
Coverage is defined as the number of sample nucleotide bases sequence aligned to a specific locus in a reference genome. The easiest way to explain this is with a real sequenced bacterial sample that has been aligned to the reference genome…
What is The Sequencing Center’s biosafety rating?
The Sequencing Center genomics lab has been certified as a BSL2 facility. To see if we can handle your organism or contribute to your research, contact us.
How long does sequencing take?
End-to-end genome sequencing varies based on the sequencing type, sample quality, and number of samples. We typically estimate turnaround between 10-15 business days for smaller projects, from the time your samples arrive in the lab to when…
How can you access your sequencing data?
When sequencing runs are finished, we automatically upload FastQ files to a client's Box.com account.
These files are typically found in the directory:
ClientName-> Reports-> ReportID-> FastQ
The FastQ files in this directory…
What is multiplex sequencing?
Some researchers may only have one or two samples that need to be sequenced. However, with the price of consumables and facility overhead, sequencing a single sample is often cost-prohibitive in normal sequencing lab settings. Multiplex sequencing…