What is cluster density?
Cluster density refers to the number of DNA clusters per unit area on an Illumina Next Generation sequencing instrument flowcell. The unit of measure is "K/mm2", or thousands of DNA clusters per square milimeter.
This is a critically important…
What is multiplexing?
Multiplexing (pooling) is a technique used to simultaneously analyze multiple samples in a single Next Generation sequencing run. It involves the use of unique index (barcode) sequences that are added to each sample before sequencing. These…
Do you accept extracted DNA for HLA Typing?
We can accept extracted DNA samples from clients for HLA Typing with the following conditions:
The DNA concentration must exceed the minimum requirement of 25 ng/uL. We prefer a higher concentration, if possible, above 30 ng/uL.
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Whole Genome Sequencing sample submission guidelines
Submitting high-quality samples is the first step to ensuring your samples pass our QC checks and receive the best sequencing results. Please review the following submission guidelines before sending in samples. If you have any questions, please…
How do we automate DNA quantitation?
In a separate Knowledge Base article we discuss manual methods for quantitating purified DNA samples. Manual quantitation is perfectly acceptable for small numbers of samples but can become prohibitively burdensome when working with larger…
How do we quantitate DNA?
DNA concentration is key. In our HLA sequencing protocols, one of the first, and most important, steps is to accurately determine the concentration of dsDNA (double-stranded DNA). This is a critical QC (quality control) check that affects…
How do you increase DNA concentration?
The critical component of a successful genome sequencing run is having sufficient DNA concentration. All of our laboratory protocols and vendor kit documentation specify minimum DNA concentration requirements. Sample DNA concentrations must…
How much DNA is contained in human blood cells?
Human blood consists of leukocytes, thrombocytes, and erythrocytes. Only leukocytes contain a cell nucleus. Thrombocytes and erythrocytes do not contain a cell nucleus. Thus, leukocytes are the most important element when estimating the…
How do you perform DNA quantitation: fluorometric vs spectrophotometric methods?
We receive a wide range of sample types in our Lab for genome sequencing. This includes human DNA, microbial DNA, bacterial DNA, bacteriophage DNA, viral DNA, yeast DNA, microbiome DNA, model and non-model organism DNA, archael DNA, and many…
HLA Typing sample submission guidelines
Submitting high-quality samples is the first step to ensuring your samples pass our QC checks and receive the best sequencing results. Please review the following submission guidelines before sending in samples. If you have any questions, please…
How long will you store my sample or extraction?
Our policy is to store all samples and extractions for up to 30 days after your run is completed, data is delivered, and we have invoiced your project.