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How to generate a complete genome with hybrid assembly

Introduction In this article we'll describe the Unicycler hybrid assembly pipeline.  Unicycler is designed primarily for de novo assembly of bacterial genomes and includes the major steps required for hybrid assembly of sequenced microbial…
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How to perform de novo assembly with PATRIC

De novo assembly is a method for generating contigs, scaffolds or complete genomes from Next Generation sequencing reads (i.e. DNA fragments).  Some researchers use the free, online bioinformatics tool PATRIC for de novo assembly.  PATRIC…