Long-Read Sequencing
Fully finished genomes from a single sample
Discover the benefits of long-read de novo assembly and long-read/short-read hybrid assembly
Short-Read Only | Long-Read Only | Hybrid Assembly (short-read + long-read) | |
---|---|---|---|
Single nucleotide polymorphism (SNP) | ✔ Yes | ✔ Yes | ✔ Yes |
Short indels (insertions / deletions) | ✔ Yes | ✔ Yes | ✔ Yes |
Copy number variations (CNV’s) | ✔ Yes | ✔ Yes | ✔ Yes |
Q-score (Phred score) > 99.99% | ✔ Yes | ✖ No | ✔ Yes |
Large structural variants | ✖ No | ✔ Yes | ✔ Yes |
Long repeat sequences | ✖ No | ✔ Yes | ✔ Yes |
Translocations | ✖ No | ✔ Yes | ✔ Yes |
Inversions | ✖ No | ✔ Yes | ✔ Yes |
Duplications | ✖ No | ✔ Yes | ✔ Yes |
Long N50 length | ✖ No | ✔ Yes | ✔ Yes |
Generate a reference genome | ✖ No | ✖ No | ✔ Yes |
You can use a combination of Nanopore long-reads, Illumina short-reads and hybrid de novo assembly to generate a novel, fully finished, complete reference genome. We can run a combination long-read/short-read project for you, including hybrid assembly, to create a unique reference genome.
If you create your own reference genome as described here, you can run sequence alignments and variant calls of multiple samples against the new reference genome. If your project includes multiple samples, you can choose one of the samples to be a reference genome. Then use long-reads, or long-read/short-read hybrid assembly, to create a reference genome from that sample. With the new genome, you can run sequence alignments and variant calls for the remaining samples against the new reference genome.
You may have a bacterial isolate sample that contains an unknown or uncertain species. With long-read sequencing alone, or a combination long-read/short-read hybrid assembly, you can generate a fully finished, complete bacterial genome. Furthermore, if there are associated plasmids in the sample, you can generate complete genomic sequences for all of the plasmids.
Bacterial strains are often identified using Multilocus Sequence Typing (MST). Illumina short-reads alone may not resolve all loci sufficiently for accurate MST. You can try long-reads alone, or long-read/short-read hybrid assembly, to use MST to identify the particular bacterial strain in your sample.
Phage and viral genomes are often quite small (< 100 kilobases). With long-read sequencing alone, you can often generate fully finished, complete genomes, without the need for hybrid long-read/short-read assembly. De novo assembly is often not required for these small genomes. And numerous samples can be multiplexed in a single sequencing run for cost-effective generation of many phage/viral genomes.
“That was a really quick turn around on the sequencing! Thank you so much!”
“This was the fastest turnaround time ever! From cells to full genome sequence in 5 days! We will definitely work together in the future. Thank you!”
“Many thanks for the quick results. The files are so easy to look at and have very thorough data readout.”
“The results look excellent. It’s been a pleasure working with you.”
The Sequencing Center is a USA-owned and operated next-generation genome sequencing company offering affordable genome sequencing and bioinformatics for research, pharmaceutical, and clinical organizations. Our services are designed for organizations performing research on bacterial, viral, and human-oriented research. Among these services, our facility offers targeted sequencing methods for research fields including inherited diseases, oncology, neurodegenerative diseases, antibiotic-resistant gene analysis, and biomarker discovery for drug development optimization.
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