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Why does DRB1/3/4/5 show ‘No typing result available’?

We use the GenDx NGSengine application for HLA typing and HLA report generation. The HLA loci DRB3, DRB4 and DRB5 are paralogs of locus DRB1.  Thus, they share a significant fraction of their genetic sequence with DRB1. The NGSengine HLA…
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What does an HLA report look like?

Our HLA reports are generated automatically by the GenDX NGSengine® software and includes information about HLA allele typing calls, allele ambiguities and relevant metadata. The exact set of loci displayed in an HLA report will depend on…
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HLA PIRCHE matching service

NGSengine can now export HLA typing results of an individual sample directly to the PIRCHE matching services website. This will facilitate laboratories to predict indirectly recognizable HLA epitopes for either hematopoietic stem cells or solid…

What is the IMGT/HLA database?

IMGT Database The IPD-IMGT/HLA database contains genetic sequence and allele data for the human major histocompatibility complex (MHC). This database is foundational for many HLA Typing algorithms and Next-Generation sequencing projects.  It…
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What does “exon mismatch” mean in HLA reports?

If you see the identifier "exon mismatch" in some alleles in our HLA reports, there may be a good explanation for it.  We'll explain briefly here.   IMGT Database The IMGT HLA database is a standard reference library for determining…
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Do you have a GenDx HLA publication list?

We vet our vendors carefully before using products in our sequencing workflows.  The Sequencing Center uses the GenDx libraries for NGS (Next Generation Sequencing)-based HLA typing.  The combination of substantial immunological research,…
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How do you read HLA reports?

We deliver detailed HLA reports to clients immediately after their HLA sequencing runs are complete. The reports include information about HLA allele typing calls, allele ambiguities, and relevant metadata. HLA Loci The full set of HLA loci…
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What are HLA ambiguous alleles?

In our CareDx TruSight Assign HLA reports, ambiguous alleles are identified with a double dash (- -). For example, "- -:01:01" indicates an ambiguity in the first field, "01:- -:01" indicates an ambiguity in the second field, and "01:01:-…
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Can you explain HLA allele nomenclature?

The standardized HLA gene and allele nomenclature is managed by the WHO Nomenclature Committee for Factors of the HLA System.  A high-level summary of their nomenclature system is reproduced here for easy reference.  A thorough and detailed…
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How long does genome sequencing take?

End-to-end genome sequencing varies based on the sequencing type, sample quality, and number of samples. We typically estimate turnaround between 10-15 business days for smaller projects, from the time your samples arrive in the lab to when…
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HLA sample requirements

We use the GenDx NGS HLA typing protocols. The sample requirements for HLA typing projects analyzing the core 11 loci are listed below. Samples that do not meet these requirements will not be rejected. The results from our internal testing with…