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What is 4-field resolution HLA typing?

HLA typing resolution refers to the level of detail to which the HLA alleles are identified. There are 4 possible fields of resolution: Antigen recognition site (1-field) -- Identifies the major antigen recognition site variants, e.g. HLA-A*02 …

What are common failures in sample collection that lead to issues in HLA Typing accuracy?

Human Leukocyte Antigen (HLA) typing is a critical process used in organ transplantation, immunology, and disease association studies. The accuracy of HLA typing is crucial to ensure correct compatibility assessment and to minimize the risk…

What is HLA Typing?

Human Leukocyte Antigen (HLA) Typing or genotyping is a process used to determine the variation between genes in individuals that code for HLA proteins in their white blood cells. This process is important as it affects the way an individual’s…
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Why does DRB1/3/4/5 show ‘No typing result available’?

We use the GenDx NGSengine application for HLA typing and HLA report generation. The HLA loci DRB3, DRB4 and DRB5 are paralogs of locus DRB1.  Thus, they share a significant fraction of their genetic sequence with DRB1. The NGSengine HLA…
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What does an HLA report look like?

Our HLA reports are generated automatically by the GenDX NGSengine® software and includes information about HLA allele typing calls, allele ambiguities and relevant metadata. The exact set of loci displayed in an HLA report will depend on…
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HLA PIRCHE matching service

NGSengine can now export HLA typing results of an individual sample directly to the PIRCHE matching services website. This will facilitate laboratories to predict indirectly recognizable HLA epitopes for either hematopoietic stem cells or solid…

What is the IMGT/HLA database?

IMGT Database The IPD-IMGT/HLA database contains genetic sequence and allele data for the human major histocompatibility complex (MHC). This database is foundational for many HLA Typing algorithms and Next-Generation sequencing projects.  It…
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What does “exon mismatch” mean in HLA reports?

If you see the identifier "exon mismatch" in some alleles in our HLA reports, there may be a good explanation for it.  We'll explain briefly here.   IMGT Database The IMGT HLA database is a standard reference library for determining…
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What version of HLA NGSengine do you use?

GenDx Reports We use GenDx NGSengine for HLA typing and HLA report generation.  The current version of NGSengine v.2.29.0.  This version of NGSengine includes the IMGT/HLA database v.3.50.0.    
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Do you have a GenDx HLA publication list?

We vet our vendors carefully before using products in our sequencing workflows.  The Sequencing Center uses the GenDx libraries for NGS (Next Generation Sequencing)-based HLA typing.  The combination of substantial immunological research,…
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How do you read HLA reports?

We deliver detailed HLA reports to clients immediately after their HLA sequencing runs are complete. The reports include information about HLA allele typing calls, allele ambiguities, and relevant metadata. HLA Loci The full set of HLA loci…
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How to read the HLA allele nomenclature

The standardized HLA gene and allele nomenclature is managed by the WHO Nomenclature Committee for Factors of the HLA System.  A high-level summary of their nomenclature system is reproduced here for easy reference.  A thorough and detailed…
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How long does sequencing take?

The turnaround time for HLA and genome sequencing varies based on the sequencing type, sample quality, and number of samples. We typically estimate turnaround between 10-15 business days for smaller projects, from the time your samples arrive…