HLA Typing Sample Requirements

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We use the Illumina TruSight HLA v2 Sequencing Panel for HLA typing runs.  The minimum sample requirements for this assay are:

  • DNA mass:  2 µg
  • DNA concentration:  20 ng/µL
  • Sample volume:  100 µL

NOTE:  If necessary, we can work with lower sample volumes and/or lower DNA concentrations, but it requires some modifications to our library prep protocols. The absolute minimums are sample volume = 50 µL and DNA concentration = 10 ng/µL.  Please contact us if you have any questions about minimum sample volumes or DNA concentration.