We use the Illumina TruSight HLA v2 Sequencing Panel for HLA typing runs. The minimum sample requirements for this assay are:
- DNA mass: 2 µg
- DNA concentration: 20 ng/µL
- Sample volume: 100 µL
NOTE: If necessary, we can work with lower sample volumes and/or lower DNA concentrations, but it requires some modifications to our library prep protocols. The absolute minimums are sample volume = 50 µL and DNA concentration = 10 ng/µL. Please contact us if you have any questions about minimum sample volumes or DNA concentration.