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We use GenDx NGSgo and NGSengine for HLA projects.  The minimum sample requirements for this HLA typing assay are:

  • DNA mass:  1,800 ng
  • DNA concentration:  25-30 ng/µL (measured by Qubit fluorometer)
  • Sample volume:  60 µL

Samples that do not meet these minimum requirements will not be run.  During our tests with samples under these values, the results frequently produced incomplete data and missing loci, so these requirements are strictly enforced.  Please contact us if you have any questions about minimum sample volumes or DNA concentration.