How do we automate DNA quantitation?

In a separate Knowledge Base article we discuss manual methods for quantitating purified DNA samples.  Manual quantitation is perfectly acceptable for small numbers of samples but can become prohibitively burdensome when working with larger…

How do we quantitate DNA?

DNA concentration is key.  In our HLA sequencing protocols, one of the first, and most important, steps is to accurately determine the concentration of dsDNA (double-stranded DNA).  This is a critical QC (quality control) check that affects…

What is index hopping?

Index hopping occurs when index (barcode) sequences initially assigned to a specific sample are incorrectly assigned to other sample(s) in a pool of samples.  The result of index hopping is the incorrect assignment of sample reads (DNA fragments)…
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How do you increase DNA concentration?

The critical component of a successful genome sequencing run is having sufficient DNA concentration. All of our laboratory protocols and vendor kit documentation specify minimum DNA concentration requirements.  Sample DNA concentrations must…
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How much DNA is contained in human blood cells?

Human blood consists of leukocytes, thrombocytes, and erythrocytes. Only leukocytes contain a cell nucleus.  Thrombocytes and erythrocytes do not contain a cell nucleus.  Thus, leukocytes are the most important element when estimating the…
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How do you perform DNA quantitation: fluorometric vs spectrophotometric methods?

We receive a wide range of sample types in our Lab for genome sequencing. This includes human DNA, microbial DNA, bacterial DNA, bacteriophage DNA, viral DNA, yeast DNA, microbiome DNA, model and non-model organism DNA, archael DNA, and many…

HLA Typing sample submission guidelines

Submitting high-quality samples is the first step to ensuring your samples pass our QC checks and receive the best sequencing results. Please review the following submission guidelines before sending in samples. If you have any questions, please…
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How long will you store my sample or extraction?

Our policy is to store all samples and extractions for up to 30 days after your run is completed, data is delivered, and we have invoiced your project.