Entries by Richard Casey

The Sequencing Center Now Offers Long-Read Sequencing Services

FOR IMMEDIATE RELEASE Fort Collins, Colorado, 10/12/2020 – The Sequencing Center, a genome sequencing service provider for biological and clinical research, announced today that it will offer long-read sequencing services to supplement its current short-read services. With this new service, the Center will provide a comprehensive suite of whole genome and targeted sequencing solutions. “We’re […]

How Did We Discover a Novel HLA Allele?

Intro One of the pleasures of owning a genomics company is assisting client researchers with new discoveries.  Recently, one of our clients completed an 11-loci, 4-field HLA typing project for a clinical trial project.  Using Illumina Next Generation Sequencing (NGS) instruments they multiplexed 48 samples for quick turnaround on a short-deadline schedule.  While most samples […]

HLA Susceptibility in SARS-CoV-2

Intro Nguyen et al. recently published a paper “Human leukocyte antigen susceptibility map for SARS-CoV-2” in the Journal of Virology  Using in-silico methods, they assessed the binding affinities of MHC Class I alleles (HLA-A, HLA-B, HLA-C) with all known SARS-CoV-2 8-12mer peptides from the full SARS-CoV-2 proteome.  Presumably, the binding affinities of MHC Class I molecules to SARS-CoV-2 […]

Cost Savings with Multiplex Samples

Multiplex Sequencing Multiplexing is a method of combining two or more samples in a single sequencing run.  Multiplexing can increase the throughput of sequencing runs and save money at the same time. Conceptually, multiplexing is a fairly straightforward process.   Fig. 1 Fig. 1 shows how (two) samples are multiplexed and demultiplexed in a sequencing […]

Gene Panel Design: ACMG Standards

The Paper Bean et al. recently published a report “Diagnostic gene sequencing panels: from design to report – a technical standard of the American College of Medical Genetics and Genomics (ACMG)” that provides technical standards for the design of gene panels.  The report and standards will be of interest to clinical researchers and others who […]

Bacteriophage DNA Extraction Protocol

We routinely perform bacteriophage whole genome sequencing.  However, we do not currently offer phage DNA extraction services.  Instead, researchers perform phage DNA extraction at their own facilities and then ship purified gDNA to our sequencing Lab.  Apparently, DNA extraction for some phage species can be challenging.  Therefore, we present the following option. Jakociune and Moodley, […]