Entries by Richard Casey

Cost Savings with Multiplex Samples

Multiplex Sequencing Multiplexing is a method of combining two or more samples in a single sequencing run.  Multiplexing can increase the throughput of sequencing runs and save money at the same time. Conceptually, multiplexing is a fairly straightforward process.   Fig. 1 Fig. 1 shows how (two) samples are multiplexed and demultiplexed in a sequencing […]

Gene Panel Design: ACMG Standards

The Paper Bean et al. recently published a report “Diagnostic gene sequencing panels: from design to report – a technical standard of the American College of Medical Genetics and Genomics (ACMG)” that provides technical standards for the design of gene panels.  The report and standards will be of interest to clinical researchers and others who […]

Bacteriophage DNA Extraction Protocol

We routinely perform bacteriophage whole genome sequencing.  However, we do not currently offer phage DNA extraction services.  Instead, researchers perform phage DNA extraction at their own facilities and then ship purified gDNA to our sequencing Lab.  Apparently, DNA extraction for some phage species can be challenging.  Therefore, we present the following option. Jakociune and Moodley, […]