Basic Bioinformatics: How to Read an HLA Report
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Episode 8: How to Read an HLA Report

Today, I'd like to review the HLA typing reports that we generate from every HLA sequencing run that we make. This is a standard report that's generated with every sample within an HLA typing run. We'll go through the different fields here and…
Basic Bioinformatics: Generate a Geneious Variant Report
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Episode 5: How to Generate a Geneious Variant Report

In this video we are going to talk about how to generate a Geneious variant call report file. Load the File We are going to assume that you already have Geneious Prime launched and running, that you have already imported your sample FastQ…
Basic Bioinformatics: Sequencing Variant Calls
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Episode 6: Sequencing Variant Calls

In a previous video we talked about alignments of bacterial sample reads against the reference genome. In this case Pseudomonas aeruginosa. What I want to do in this brief demo is show you how to save the variant calls to an Excel spreadsheet…
Basic Bioinformatics: Sequencing Coverage

Episode 7: Sequencing Coverage

In a previous video, we did an alignment of our sample reads against a reference genome, in this case pseudomonas aeruginosa.  We're using the Geneious tool to look at the alignment and variant calls.  What I wanted to do here was just briefly…
Basic Bioinformatics: Importing Bacterial Reference Genomes into Geneious
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Episode 3: Importing Bacterial Reference Genomes into Geneious

This video will show you one method of importing a bacterial reference genome into Geneious Prime. Once you have imported the reference genome, you can do things like sequence alignments, variant calls, annotations, and lots of other fun things.…
Basic Bioinformatics: Importing Illumina Files into Geneious
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Episode 2: Importing Illumina Files into Geneious

In this video we are going to show how to import Illumina paired-end FASTQ files into Geneious Prime. We have quite a few clients who used Geneious for bioinformatics data analysis and will often ask "What's the correct way to import paired-end…
Basic Bioinformatics: Microbial DNA Variants Pipeline in Geneious

Episode 4: Microbial DNA Variants Pipeline in Geneious

In this video we will go step-by-step through a microbial DNA variants pipeline using Genius Prime. This is the main dashboard screen and I have already loaded up paired-end reads, both the forward reads and the reverse reads, into a single…
Basic bioinformatics: Overview of a Microbial DNA Variants Pipeline

Episode 1: Overview of a Microbial DNA Variants Pipeline

This video is a high level review of a bioinformatics pipeline for microbial DNA variants.  We won't go into detail about the rationale for each step or explain it step in detail since this is meant to just review the process. We will assume…